General Cell Based Assays
Cayman Chemical SphngosIn KInase 2humn25ug
Source: Active recombinant N-terminal His-tagged protein expressed in insect cells • Amino acids: 2-654 (full length) • MW: 72.9 kDa
Meso Scale Discovery RPLEX Hu Cathepsin S TTL ASY
The R-PLEX Hu Cathepsin S Total Assay Kit from Meso Scale Discovery is a multiplex immunoassay that quantitatively measures human Cathepsin S in cell and tissue lysates This assay utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity a wide dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody Read Buffer and Calibrator This assay allows for the simple and rapid measurement of Cathepsin S across multiple samples
Eurofins Discoverx PathHunter eXpress GCGR Total Internalization Assay, 10pl Kit. (93-0820E1CP0L)
The PathHunter GCGR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GCGR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged GCGR and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GCGR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter MCHR1 Bioassay Kit, 10-Plate. (93-0940Y3-00150)
The PathHunter MCHR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility and faster implementation from characterization to lot release. This bioassay has been optimized for a 96-well plate format. The protocol can be optimized further for running the assay in a high-throughput 384-well plate format. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter™ Human AVPR2 Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human AVPR2 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure AVPR2 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress GPR157 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0414E2ACP2M)
The PathHunter GPR157 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR157 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR157 and EA-tagged β-Arrestin-2. Activation of GPR157 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Liraglutide Bioassay Kit, 10-Plate. (95-0062Y2-00100)
The cAMP Hunter Liraglutide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Liraglutide based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Bioassay Systems QuantiChrom™ Magnesium Assay Kit. For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism.
For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).
Eurofins Discoverx cAMP Hunter eXpress GRM4 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0117E2CP24M)
The cAMP Hunter GRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. L-glutamate) based activation of GRM4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GRM4, a naturally Gi/Go coupled GPCR. Upon activation of GRM4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Triglyceride Standard
Triglyceride Standard
The product is subject to the following: Abcam Restricted Use Statement
Bioassay Systems EnzyChrom™ AF HDL and LDL/VLDL Assay Kit. For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. Method: OD570nm, or FL530/585nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 mg/dL.
Abcam Free Fatty Acid Assay Kit -¿Quantification
Free Fatty Acid Assay Kit -¿Quantification
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter GLP2R Bioassay Kit, 2-Plate. (93-0572Y2-00133)
The PathHunter GLP2R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. GLP2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress MCHR1 Total Internalization Assay, 10pl Kit. (93-0947E3CP5L)
The PathHunter MCHR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of MCHR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged MCHR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MCHR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc Lipid Extraction Kit (Chloroform-Free), 50 preps
Traditional lipid extraction by the Folch method is tedious and uses toxic chloroform as the key solvent. In addition, the extracted lipids end up in the bottom layer which is inconvenient. Cell Biolabs' Lipid Extraction Kit (Chloroform-Free) uses an organic solvent system that results in the organic phase being on the top, making it easier to use while eliminating the need for chloroform. Each kit provides sufficient reagents for 50 preps based on a 100µL sample size.